Fig 1: Validation of possible PLD targets by RT-qPCR. Gene expression of 8 to 10 selected targets correlated with SWATH-MS data. RT-qPCRs of up-regulated selected p14 target annexin A2 (Anxa2) (a); up-regulated p12 targets amyloid p component, serum (Apcs, SAMP gene), fibrinogen-like 1 (Fgl1), fibrinogen beta chain (Fgb), hemopexin (Hpx), fibrinogen alpha chain (Fga), fibrinogen gamma chain (Fgg) (b), integrin linked kinase (Ilk) and S100 calcium binding protein A9 (S100a9) (c); and down-regulated p12 target hydroxyacid oxidase 2 (Hao2) (d). n = 6 was used for each protein group. Gapdh was used as a housekeeping gene. Bars represent means ± SEM. Student’s t-test with two-tail was used and a value of p < 0.05 was considered significant. ns: not significant (p ≥ 0.05), * p < 0.05, ** p < 0.01, *** p < 0.001.